Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker

This search aimed to investigate the genetic diversity of 17 different phenotypes collected from a Lepidium sativum plant from different regions of Iran province and investigate kinship relationships. DNA was extracted from leaf samples using the CTAB method. To evaluate 8 RAPD primers’ genetic diversity, a total of 70 bands were amplified, 47 polymorphic bands and 23 single bands, with the highest number of OPJ-10 primers with 13 bands and the lowest number of OPA-09 and OPB-01 primers with five bands. The OPJ-10 primer had the highest EMR values of 10.8 and β of 0.9, and this value was zero in the EMR, β primers OPB-01 and OPA-09. In cluster analysis using the UPGMA method (with a genetic distance of 0.84), the six studied populations were classified. The highest genetic distance was obtained between Kerman and Joupar populations (with a genetic similarity of 0.69), which can be used to produce hybrid cultivars and heterosis productivity.